Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

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dc.contributor.authorLichocka, Malgorzata
dc.contributor.authorRymaszewski, Wojciech
dc.contributor.authorMorgiewicz, Karolina
dc.contributor.authorBarymow-Filoniuk, Izabela
dc.contributor.authorChlebowski, Aleksander
dc.contributor.authorSobczak, Miroslaw
dc.contributor.authorSamuel, Marcus A
dc.contributor.authorSchmelzer, Elmon
dc.contributor.authorKrzymowska, Magdalena
dc.contributor.authorHennig, Jacek
dc.date.accessioned2018-09-26T12:04:36Z
dc.date.available2018-09-26T12:04:36Z
dc.date.issued2018-09-06
dc.date.updated2018-09-26T12:04:36Z
dc.description.abstractAbstract Background Pollen development is a strictly controlled post-meiotic process during which microspores differentiate into microgametophytes and profound structural and functional changes occur in organelles. Annexin 5 is a calcium- and lipid-binding protein that is highly expressed in pollen grains and regulates pollen development and physiology. To gain further insights into the role of ANN5 in Arabidopsis development, we performed detailed phenotypic characterization of Arabidopsis plants with modified ANN5 levels. In addition, interaction partners and subcellular localization of ANN5 were analyzed to investigate potential functions of ANN5 at cellular level. Results Here, we report that RNAi-mediated suppression of ANN5 results in formation of smaller pollen grains, enhanced pollen lethality, and delayed pollen tube growth. ANN5 RNAi knockdown plants also displayed aberrant development during the transition from the vegetative to generative phase and during embryogenesis, reflected by delayed bolting time and reduced embryo size, respectively. At the subcellular level, ANN5 was delivered to the nucleus, nucleolus, and cytoplasm, and was frequently localized in plastid nucleoids, suggesting a likely role in interorganellar communication. Furthermore, ANN5-YFP co-immunoprecipitated with RABE1b, a putative GTPase, and interaction in planta was confirmed in plastidial nucleoids using FLIM-FRET analysis. Conclusions Our findings let us to propose that ANN5 influences basal cell homeostasis via modulation of plastid activity during pollen maturation. We hypothesize that the role of ANN5 is to orchestrate the plastidial and nuclear genome activities via protein-protein interactions however not only in maturing pollen but also during the transition from the vegetative to the generative growth and seed development.
dc.identifier.citationBMC Plant Biology. 2018 Sep 06;18(1):183
dc.identifier.doihttps://doi.org/10.1186/s12870-018-1405-3
dc.identifier.urihttp://hdl.handle.net/1880/107928
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dc.titleNucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation
dc.typeJournal Article
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