Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
| dc.contributor.author | Toms, Derek | |
| dc.contributor.author | Pan, Bo | |
| dc.contributor.author | Bai, Yinshan | |
| dc.contributor.author | Li, Julang | |
| dc.date.accessioned | 2021-04-25T00:02:36Z | |
| dc.date.available | 2021-04-25T00:02:36Z | |
| dc.date.issued | 2021-04-20 | |
| dc.date.updated | 2021-04-25T00:02:36Z | |
| dc.description.abstract | Abstract Nuclear small RNAs have emerged as an important subset of non-coding RNA species that are capable of regulating gene expression. A type of small RNA, microRNA (miRNA) have been shown to regulate development of the ovarian follicle via canonical targeting and translational repression. Little has been done to study these molecules at a subcellular level. Using cell fractionation and high throughput sequencing, we surveyed cytoplasmic and nuclear small RNA found in the granulosa cells of the pig ovarian antral preovulatory follicle. Bioinformatics analysis revealed a diverse network of small RNA that differ in their subcellular distribution and implied function. We identified predicted genomic DNA binding sites for nucleus-enriched miRNAs that may potentially be involved in transcriptional regulation. The small nucleolar RNA (snoRNA) SNORA73, known to be involved in steroid synthesis, was also found to be highly enriched in the cytoplasm, suggesting a role for snoRNA species in ovarian function. Taken together, these data provide an important resource to study the small RNAome in ovarian follicles and how they may impact fertility. | |
| dc.identifier.citation | Journal of Ovarian Research. 2021 Apr 20;14(1):54 | |
| dc.identifier.doi | https://doi.org/10.1186/s13048-021-00802-3 | |
| dc.identifier.uri | http://hdl.handle.net/1880/113315 | |
| dc.language.rfc3066 | en | |
| dc.rights.holder | The Author(s) | |
| dc.title | Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth | |
| dc.type | Journal Article |