Quantum dot interactions and flow effects in angiogenic zebrafish (Danio rerio) vessels and human endothelial cells

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dc.contributor.authorJiang, Xiao-Yu
dc.contributor.authorSarsons, Christopher D
dc.contributor.authorGomez-Garcia, M Juliana
dc.contributor.authorCramb, David T
dc.contributor.authorRinker, Kristina D
dc.contributor.authorChilds, Sarah J
dc.date.accessioned2022-08-24T22:20:06Z
dc.date.available2022-08-24T22:20:06Z
dc.date.issued2017-01
dc.description.abstractNanoparticle (NP) interactions with biological tissues are affected by the size, shape and surface chemistry of the NPs. Here we use in vivo (zebrafish) and in vitro (HUVEC) models to investigate association of quantum dots (QDs) with endothelial cells and the effect of fluid flow. After injection into the developing zebrafish, circulating QDs associate with endothelium and penetrate surrounding tissue parenchyma over time. Amino-functionalized QDs cluster, interact with cells, and clear more rapidly than carboxy-functionalized QDs in vivo, highlighting charge influences. QDs show stronger accumulation in slow-flowing, small caliber venous vessels than in fast-flowing high caliber arterial vessels. Parallel-plate flow experiments with HUVEC support these findings, showing reduced QD-EC association with increasing flow. In vivo, flow arrest after nanoparticle injection still results in venous accumulation at 18 h. Overall our results suggest that both QD charge and blood flow modulate particle-endothelial cell interactions.en_US
dc.description.grantingagencyCanadian Institutes of Health Research (CIHR)en_US
dc.identifier.citationJiang XY, et al, Quantum dot interactions and flow effects in angiogenic zebrafish (Danio rerio) vessels and human endothelial cells. Nanomedicine: NBM 2017;13:999-1010, http://dx.doi.org/10.1016/j.nano.2016.12.008en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.nano.2016.12.008en_US
dc.identifier.urihttp://hdl.handle.net/1880/115132
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.publisher.departmentBiochemistry & Molecular Biologyen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.hasversionacceptedVersionen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://www.elsevier.com/journals/nanomedicine-nanotechnology-biology-and-medicine/1549-9634/open-access-optionsen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nd/4.0en_US
dc.titleQuantum dot interactions and flow effects in angiogenic zebrafish (Danio rerio) vessels and human endothelial cellsen_US
ucalgary.item.requestcopytrueen_US
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