Lead Interactions Affect the Fluidity and Lateral Organization of Complex Lipid Membranes
Date
2018-03-26
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Abstract
Elevated levels of the heavy metal lead are associated with various toxic effects which may also be due to interactions with lipid membranes. Bilayer and monolayer techniques were used to monitor lead interactions with biomimetic and polar lipid extracts. Anionic and zwitterionic lipids as well as saturated and monounsaturated fatty acids were compared. Permeabilization of model membranes by lead was assessed by quantifying fluorescent dye release, whereby leakage readily occurred upon Pb2+ incubation. Fluidity and phase transition changes were investigated by laurdan generalized polarization and dynamic light scattering. Electrostatic lipid targeting by lead induced membrane
rigidification which varied with the negative charges within lipid structures. Lead effects on the lateral organization of monolayers were characterized by surface pressure- and surface potential – area isotherms and Brewster angle microscopy. Pb – induced domain formation depends on the number and localization of the phosphate groups on various phosphatidylinositols.
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Keywords
Lipids, Fluorescence Spectroscopy, Liposomes, Laurdan, Monolayers, Brewster Angle Microscopy, Lead, Langmuir Trough
Citation
Mundle, R. L. (2018). Lead Interactions Affect the Fluidity and Lateral Organization of Complex Lipid Membranes (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/31755