Lead Interactions Affect the Fluidity and Lateral Organization of Complex Lipid Membranes

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dc.contributor.advisorPrenner, Elmar J.
dc.contributor.authorMundle, Robyn Louise
dc.contributor.committeememberGailer, Jürgen G.
dc.contributor.committeememberTurner, Raymond Joseph
dc.contributor.committeememberNoskov, Sergei Yu
dc.date2018-06
dc.date.accessioned2018-04-03T21:45:40Z
dc.date.available2018-04-03T21:45:40Z
dc.date.issued2018-03-26
dc.description.abstractElevated levels of the heavy metal lead are associated with various toxic effects which may also be due to interactions with lipid membranes. Bilayer and monolayer techniques were used to monitor lead interactions with biomimetic and polar lipid extracts. Anionic and zwitterionic lipids as well as saturated and monounsaturated fatty acids were compared. Permeabilization of model membranes by lead was assessed by quantifying fluorescent dye release, whereby leakage readily occurred upon Pb2+ incubation. Fluidity and phase transition changes were investigated by laurdan generalized polarization and dynamic light scattering. Electrostatic lipid targeting by lead induced membrane rigidification which varied with the negative charges within lipid structures. Lead effects on the lateral organization of monolayers were characterized by surface pressure- and surface potential – area isotherms and Brewster angle microscopy. Pb – induced domain formation depends on the number and localization of the phosphate groups on various phosphatidylinositols.en_US
dc.identifier.citationMundle, R. L. (2018). Lead Interactions Affect the Fluidity and Lateral Organization of Complex Lipid Membranes (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/31755en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/31755
dc.identifier.urihttp://hdl.handle.net/1880/106463
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyScience
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectLipids
dc.subjectFluorescence Spectroscopy
dc.subjectLiposomes
dc.subjectLaurdan
dc.subjectMonolayers
dc.subjectBrewster Angle Microscopy
dc.subjectLead
dc.subjectLangmuir Trough
dc.subject.classificationBiochemistryen_US
dc.titleLead Interactions Affect the Fluidity and Lateral Organization of Complex Lipid Membranes
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.checklistI confirm that I have submitted all of the required forms to Faculty of Graduate Studies.en_US
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