Intra-articular injection of synovial mesenchymal stem cells improves cartilage repair in a mouse injury model

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dc.contributor.authorMak, J.
dc.contributor.authorJablonski, C. L.
dc.contributor.authorLeonard, C. A.
dc.contributor.authorDunn, Jeffery F.
dc.contributor.authorRaharjo, E.
dc.contributor.authorMatyas, J. R.
dc.contributor.authorBiernaskie, J.
dc.contributor.authorKrawetz, R. J.
dc.date.accessioned2017-03-13T21:52:21Z
dc.date.available2017-03-13T21:52:21Z
dc.date.issued2016-03-17
dc.description.abstractControversy remains whether articular cartilage has an endogenous stem/progenitor cell population, since its poor healing capacity after injury can lead to diseases such as osteoarthritis. In the joint environment there are mesenchymal stem/progenitor cells (MSCs) in the synovial membrane and synovial fluid that can differentiate into cartilage, but it is still under debate if these cells contribute to cartilage repair in vivo. In this study, we isolated a Sca-1 positive, chondrogenesis capable population of mouse synovial MSCs from C57BL6 and MRL/MpJ “super-healer” strains. Intra-articular injection of Sca-1 + GFP + synovial cells from C57BL6 or MRL/MpJ into C57BL6 mice following cartilage injury led to increased cartilage repair by 4 weeks after injury. GFP expression was detected in the injury site at 2 weeks, but not 4 weeks after injury. These results suggest that synovial stem/progenitor cells, regardless of strain background, have beneficial effects when injected into an injured joint. MSCs derived from MRL/MpJ mice did not promote an increased repair capacity compared to MSCs derived from non-healing C57BL6 controls; however, MRL/MpJ MSCs were observed within the defect area at the time points examined, while C57BL6 MSCs were not.en_US
dc.description.grantingagencyCanadian Institutes of Health Research; Alberta Innovates Health Solution (AIHS) Osteoarthritis Team Grant; Canada Foundation for Innovation (CFI), Canada Research Chairs (CRC) Program, NSERC Createen_US
dc.description.refereedYesen_US
dc.identifier.citationMak, J. et al. Intra-articular injection of synovial mesenchymal stem cells improves cartilage repair in a mouse injury model. Sci. Rep. 6, 23076; doi: 10.1038/srep23076 (2016).en_US
dc.identifier.doi10.1038/srep23076
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/33506
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1880/51851
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.publisher.corporateUniversity of Calgary
dc.publisher.departmentSurgeryen_US
dc.publisher.facultyMedicineen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.titleIntra-articular injection of synovial mesenchymal stem cells improves cartilage repair in a mouse injury modelen_US
dc.typejournal article
thesis.degree.disciplineRadiology / Physiology / Clinical Neurosciences
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