Immunolocalization and lc-ms/ms proteomics reveal in interplay between sieve elements and laticifers in the biosynthesis of morphine in opium poppy

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dc.contributor.advisorFacchini, Peter J.
dc.contributor.authorOnoyovwi, Akpevwe
dc.date.accessioned2017-12-18T22:34:55Z
dc.date.available2017-12-18T22:34:55Z
dc.date.issued2012
dc.descriptionBibliography: p. 87-99en
dc.description.abstractOpium poppy produces a group of natural products called benzylisoquinoline alkaloids (BIAs) such as morphine and codeine. BIAs have been shown to be synthesized in the phloem specifically in sieve elements, and accumulate in the latex, which is the cytoplasm of specialized secretory cells called laticifers. This project aimed to identify the cell types involved morphine biosynthesis via immunolocalization of six biosynthetic enzymes and LC-MS/MS proteomics analysis on latex and whole stem proteins. This resulted in the localization of codeine 0-demethylase (CODM), the last enzyme involved in morphine biosynthesis in laticifers, different from the other biosynthetic enzymes. This indicates a biosynthetic role for the laticifers. The proteomes showed the presence of morphine biosynthetic enzymes and other BIA biosynthetic enzymes. A new model is proposed where both cell types function in synthesizing morphine, with the penultimate steps occurring predominantly in laticifers. It also suggests the need for intercellular transport of alkaloid.
dc.format.extentxiii, 229 leaves : ill. ; 30 cm.en
dc.identifier.citationOnoyovwi, A. (2012). Immunolocalization and lc-ms/ms proteomics reveal in interplay between sieve elements and laticifers in the biosynthesis of morphine in opium poppy (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4926en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4926
dc.identifier.urihttp://hdl.handle.net/1880/105927
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleImmunolocalization and lc-ms/ms proteomics reveal in interplay between sieve elements and laticifers in the biosynthesis of morphine in opium poppy
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2115 627942985
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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