Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells

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dc.contributor.authorLi, Xiao-Hong
dc.contributor.authorMcGrath, Kristine C. Y.
dc.contributor.authorTran, Van H.
dc.contributor.authorLi, Yi-Ming
dc.contributor.authorMandadi, Sravan
dc.contributor.authorDuke, Colin C.
dc.contributor.authorHeather, Alison K.
dc.contributor.authorRoufogalis, Basil D.
dc.date.accessioned2018-09-27T11:40:23Z
dc.date.available2018-09-27T11:40:23Z
dc.date.issued2013-07-17
dc.date.updated2018-09-27T11:40:23Z
dc.description.abstractCalcium signals in hepatocytes control cell growth, proliferation, and death. Members of the transient receptor potential (TRP) cation channel superfamily are candidate calcium influx channels. NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival. Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1) in neurones. In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements. We found that S-[6]-gingerol induced a transient rise in in HuH-7 cells. The increase in induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine. Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of by TRPV1 agonist capsaicin and its inhibition by capsazepine. We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells. Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1. The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.
dc.description.versionPeer Reviewed
dc.identifier.citationXiao-Hong Li, Kristine C. Y. McGrath, Van H. Tran, et al., “Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 951758, 7 pages, 2013. doi:10.1155/2013/951758
dc.identifier.doihttps://doi.org/10.1155/2013/951758
dc.identifier.urihttp://hdl.handle.net/1880/108257
dc.identifier.urihttps://doi.org/10.11575/PRISM/45208
dc.language.rfc3066en
dc.rights.holderCopyright © 2013 Xiao-Hong Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleIdentification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells
dc.typeJournal Article
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