Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair

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dc.contributor.authorChijimatsu, Ryota
dc.contributor.authorIkeya, Makoto
dc.contributor.authorYasui, Yukihiko
dc.contributor.authorIkeda, Yasutoshi
dc.contributor.authorEbina, Kosuke
dc.contributor.authorMoriguchi, Yu
dc.contributor.authorShimomura, Kazunori
dc.contributor.authorHart, David A
dc.contributor.authorYoshikawa, Hideki
dc.contributor.authorNakamura, Norimasa
dc.date.accessioned2018-09-27T11:16:38Z
dc.date.available2018-09-27T11:16:38Z
dc.date.issued2017-05-18
dc.date.updated2018-09-27T11:16:38Z
dc.description.abstractMesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) are a promising cell source for the repair of skeletal disorders. Recently, neural crest cells (NCCs) were reported to be effective for inducing mesenchymal progenitors, which have potential to differentiate into osteochondral lineages. Our aim was to investigate the feasibility of MSC-like cells originated from iPSCs via NCCs for osteochondral repair. Initially, MSC-like cells derived from iPSC-NCCs (iNCCs) were generated and characterized in vitro. These iNCC-derived MSC-like cells (iNCMSCs) exhibited a homogenous population and potential for osteochondral differentiation. No upregulation of pluripotent markers was detected during culture. Second, we implanted iNCMSC-derived tissue-engineered constructs into rat osteochondral defects without any preinduction for specific differentiation lineages. The implanted cells remained alive at the implanted site, whereas they failed to repair the defects, with only scarce development of osteochondral tissue in vivo. With regard to tumorigenesis, the implanted cells gradually disappeared and no malignant cells were detected throughout the 2-month follow-up. While this study did not show that iNCMSCs have efficacy for repair of osteochondral defects when implanted under undifferentiated conditions, iNCMSCs exhibited good chondrogenic potential in vitro under appropriate conditions. With further optimization, iNCMSCs may be a new source for tissue engineering of cartilage.
dc.description.versionPeer Reviewed
dc.identifier.citationRyota Chijimatsu, Makoto Ikeya, Yukihiko Yasui, et al., “Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair,” Stem Cells International, vol. 2017, Article ID 1960965, 18 pages, 2017. doi:10.1155/2017/1960965
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/33019
dc.identifier.urihttp://dx.doi.org/10.1155/2017/1960965
dc.identifier.urihttp://hdl.handle.net/1880/108083
dc.language.rfc3066en
dc.rights.holderCopyright © 2017 Ryota Chijimatsu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleCharacterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair
dc.typeJournal Article
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