Identification of a molecular gating determinant within the carboxy terminal region of Cav3.3 T-type channels
| dc.contributor.author | Jurkovicova-Tarabova, Bohumila | |
| dc.contributor.author | Cmarko, Leos | |
| dc.contributor.author | Rehak, Renata | |
| dc.contributor.author | Zamponi, Gerald W | |
| dc.contributor.author | Lacinova, Lubica | |
| dc.contributor.author | Weiss, Norbert | |
| dc.date.accessioned | 2019-04-14T00:03:11Z | |
| dc.date.available | 2019-04-14T00:03:11Z | |
| dc.date.issued | 2019-04-08 | |
| dc.date.updated | 2019-04-14T00:03:11Z | |
| dc.description.abstract | Abstract The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Our data reveal that the proximal region of the carboxy terminus contains a structural determinant essential for shaping several gating aspects of Cav3.3 channels, including voltage-dependence of activation and inactivation, inactivation kinetics, and coupling between the voltage sensing and the pore opening of the channel. Altogether, our data are consistent with a model in which the carboxy terminus stabilizes the channel in a closed state. | |
| dc.identifier.citation | Molecular Brain. 2019 Apr 08;12(1):34 | |
| dc.identifier.doi | https://doi.org/10.1186/s13041-019-0457-0 | |
| dc.identifier.uri | http://hdl.handle.net/1880/110158 | |
| dc.language.rfc3066 | en | |
| dc.rights.holder | The Author(s). | |
| dc.title | Identification of a molecular gating determinant within the carboxy terminal region of Cav3.3 T-type channels | |
| dc.type | Journal Article |