Critically Re-evaluating Carbohydrate Metabolism in Pseudomonas aeruginosa
Date
2019-09-19
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Abstract
Several studies have linked carbon metabolism to a variety of different factors in P. aeruginosa. For example, the production of gluconate and 2-ketogluconate has been linked to exotoxin production, antibiotic resistance, and iron acquisition. However, glucose catabolism is known to participate in P. aeruginosa aerobic respiration. We hypothesize that, rather than factors such as iron acquisition, P. aeruginosa gluconate and 2-ketogluconate production is instead driven by the energetic needs of the cell. We developed a targeted LC-MS method and analyzed P. aeruginosa glucose metabolism production in a variety of limiting conditions. From our data, we made a model of P. aeruginosa metabolism where energy production is decoupled from biomass generation via periplasmic glucose oxidation. This model introduces a regulatory mechanism of carbon catabolism that explains the production of gluconate and 2-ketogluconate through cellular energy demands.
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Keywords
Metabolism, Metabolomics, Pseudomonas aeruginosa, Glucose, LC-MS, Entner-Doudoroff Pathway, Gluconate, 2-ketogluconate
Citation
Nguyen, A. (2019). Critically Re-evaluating Carbohydrate Metabolism in Pseudomonas aeruginosa (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.