Critically Re-evaluating Carbohydrate Metabolism in Pseudomonas aeruginosa

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dc.contributor.advisorLewis, Ian A.
dc.contributor.authorNguyen, Austin
dc.contributor.committeememberStorey, Douglas G.
dc.contributor.committeememberHarrison, Joe J.
dc.contributor.committeememberDeVinney, Rebekah
dc.date2019-11
dc.date.accessioned2019-09-23T15:18:52Z
dc.date.available2019-09-23T15:18:52Z
dc.date.issued2019-09-19
dc.description.abstractSeveral studies have linked carbon metabolism to a variety of different factors in P. aeruginosa. For example, the production of gluconate and 2-ketogluconate has been linked to exotoxin production, antibiotic resistance, and iron acquisition. However, glucose catabolism is known to participate in P. aeruginosa aerobic respiration. We hypothesize that, rather than factors such as iron acquisition, P. aeruginosa gluconate and 2-ketogluconate production is instead driven by the energetic needs of the cell. We developed a targeted LC-MS method and analyzed P. aeruginosa glucose metabolism production in a variety of limiting conditions. From our data, we made a model of P. aeruginosa metabolism where energy production is decoupled from biomass generation via periplasmic glucose oxidation. This model introduces a regulatory mechanism of carbon catabolism that explains the production of gluconate and 2-ketogluconate through cellular energy demands.en_US
dc.identifier.citationNguyen, A. (2019). Critically Re-evaluating Carbohydrate Metabolism in Pseudomonas aeruginosa (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/37096
dc.identifier.urihttp://hdl.handle.net/1880/111034
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectMetabolismen_US
dc.subjectMetabolomicsen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectGlucoseen_US
dc.subjectLC-MSen_US
dc.subjectEntner-Doudoroff Pathwayen_US
dc.subjectGluconateen_US
dc.subject2-ketogluconateen_US
dc.subject.classificationMicrobiologyen_US
dc.subject.classificationBiochemistryen_US
dc.titleCritically Re-evaluating Carbohydrate Metabolism in Pseudomonas aeruginosaen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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