Hairpin Telomere Resolvases

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dc.contributor.authorKobryn, Kerri
dc.contributor.authorChaconas, George
dc.date.accessioned2021-04-23T19:13:15Z
dc.date.available2021-04-23T19:13:15Z
dc.date.issued2014-11-24
dc.description.abstractCovalently closed hairpin ends, also known as hairpin telomeres, provide an unusual solution to the end replication problem. The hairpin telomeres are generated from replication intermediates by a process known as telomere resolution. This is a DNA breakage and reunion reaction promoted by hairpin telomere resolvases (also referred to as protelomerases) found in a limited number of phage and bacteria. The reaction promoted by these enzymes is a chemically isoenergetic two-step transesterification without a requirement for divalent metal ions or high-energy cofactors and uses an active site and mechanism similar to that for type IB topoisomerases and tyrosine recombinases. The small number of unrelated telomere resolvases characterized to date all contain a central, catalytic core domain with the active site, but in addition carry variable C- and N-terminal domains with different functions. Similarities and differences in the structure and function of the telomere resolvases are discussed. Of particular interest are the properties of the Borrelia telomere resolvases, which have been studied most extensively at the biochemical level and appear to play a role in shaping the unusual segmented genomes in these organisms and, perhaps, to play a role in recombinational events.en_US
dc.description.grantingagencyCanadian Institutes of Health Research (CIHR)en_US
dc.identifier.citationCraig, Nancy L. and Chandler, Michael and Gellert, Martin and Lambowitz, Alan M. and Rice, Phoebe A. and Sandmeyer, Suzanne B.(ed). 2015. Mobile DNA IIIen_US
dc.identifier.doihttp://dx.doi.org/10.1128/microbiolspec.MDNA3-0023-2014en_US
dc.identifier.grantnumber53086en_US
dc.identifier.urihttp://hdl.handle.net/1880/113309
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.publisher.departmentBiochemistry & Molecular Biologyen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.hasversionacceptedVersionen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.institutionUniversity of Saskatchewanen_US
dc.publisher.policyhttps://journals.asm.org/content/statement-author-rightsen_US
dc.titleHairpin Telomere Resolvasesen_US
dc.typebook parten_US
ucalgary.item.requestcopyfalseen_US
ucalgary.scholar.levelFacultyen_US
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