Plant Defense Responses in Opium Poppy Cell Cultures Revealed by Liquid Chromatography-Tandem Mass Spectrometry Proteomics

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dc.contributor.authorZulak, Katherine G.
dc.contributor.authorKhan, Morgan F.
dc.contributor.authorAlcantara, Joenel
dc.contributor.authorSchriemer, David
dc.contributor.authorFacchini, Peter J.
dc.date.accessioned2017-08-17T20:56:57Z
dc.date.available2017-08-17T20:56:57Z
dc.date.issued2008-08-05
dc.description.abstractOpium poppy (Papaver somniferum) produces a diverse array of bioactive benzylisoquinoline alkaloids, including the narcotic analgesic morphine and the antimicrobial agent sanguinarine. In contrast to the plant, cell cultures of opium poppy do not accumulate alkaloids constitutively but produce sanguinarine in response to treatment with certain fungal-derived elicitors. The induction of sanguinarine biosynthesis provides a model platform to characterize the regulation of benzylisoquinoline alkaloid pathways and other defense responses. Proteome analysis of elicitor-treated opium poppy cell cultures by two-dimensional denaturing-polyacrylamide gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry facilitated the identification of 219 of 340 protein spots based on peptide fragment fingerprint searches of a combination of databases. Of the 219 hits, 129 were identified through pre-existing plant proteome databases, 63 were identified by matching predicted translation products in opium poppy-expressed sequence tag databases, and the remainder shared evidence from both databases. Metabolic enzymes represented the largest category of proteins and included S-adenosylmethionine synthetase, several glycolytic, and a nearly complete set of tricarboxylic acid cycle enzymes, one alkaloid, and several other secondary metabolic enzymes. The abundance of chaperones, heat shock proteins, protein degradation factors, and pathogenesis-related proteins provided a comprehensive proteomics view on the coordination of plant defense responses. Qualitative comparison of protein abundance in control and elicitor-treated cell cultures allowed the separation of induced and constitutive or suppressed proteins. DNA microarrays were used to corroborate increases in protein abundance with a corresponding induction in cognate transcript levels.en_US
dc.description.grantingagencyNatural Sciences and Engineering Research Council of Canada Discovery and Strategic Grants (to P. J. F.).en_US
dc.description.refereedYesen_US
dc.description.sponsorshipThis work was supported by funds provided by Natural Sciences and Engineering Research Council of Canada Discovery and Strategic Grants (to P. J. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.en_US
dc.identifier.citationZulak, K. G., Khan, M. F., Alcantara, J., Schriemer, D. C., & Facchini, P. J. (2009). Plant defense responses in opium poppy cell cultures revealed by liquid chromatography-tandem mass spectrometry proteomics. Molecular & Cellular Proteomics, 8(1), 86-98. doi:10.1074/mcp.M800211-MCP200en_US
dc.identifier.doi10.1074/mcp.M800211-MCP200
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/33813
dc.identifier.urihttp://hdl.handle.net/1880/52193
dc.language.isoenen_US
dc.publisherMolecular & Cellular Proteomicsen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.urlhttp://www.mcponline.orgen_US
dc.subjectAlkaloidsen_US
dc.subjectBenzophenanthridinesen_US
dc.subjectBotrytisen_US
dc.subjectCell Culture Techniquesen_US
dc.subjectChromatography, Liquiden_US
dc.subjectDNA, Complementaryen_US
dc.subjectElectrophoresis, Gel, Two-Dimensionalen_US
dc.subjectEnzyme Inductionen_US
dc.subjectExpressed Sequence Tagsen_US
dc.subjectGene Expression Regulation, Planten_US
dc.subjectIsoquinolinesen_US
dc.subjectMolecular Sequence Dataen_US
dc.subjectPapaveren_US
dc.subjectPlant Proteinsen_US
dc.subjectProteomicsen_US
dc.subjectRNA, Messengeren_US
dc.subjectTandem Mass Spectrometryen_US
dc.subjectPapaver somniferumen_US
dc.titlePlant Defense Responses in Opium Poppy Cell Cultures Revealed by Liquid Chromatography-Tandem Mass Spectrometry Proteomicsen_US
dc.typejournal article
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