The Cystic Fibrosis Microbiome and its Association with Incident Infections with Mycobacteroides (Mycobacterium) abscessus
| dc.contributor.advisor | Parkins, Michael | |
| dc.contributor.author | Bharadwaj, Lalit | |
| dc.contributor.committeemember | Storey, Douglas | |
| dc.contributor.committeemember | Arrieta, Marie-Claire | |
| dc.contributor.committeemember | Surette, Michael | |
| dc.date | 2021-06 | |
| dc.date.accessioned | 2021-05-21T15:06:09Z | |
| dc.date.available | 2021-05-21T15:06:09Z | |
| dc.date.issued | 2021-05-13 | |
| dc.description.abstract | Infection with M. abscessus complex (MABC) is increasingly detected within CF populations. MABC infection has been associated with exaggerated lung function decline and poses significant treatment complexities. We performed a retrospective case-control study of twenty-one patients with MABC infection matching each to two randomly identified age (+/-2 yrs) and gender-matched uninfected controls. Total genomic DNA from sputum was extracted, amplified and Illumina MiSeq paired-end sequencing of the hypervariable V3 region of the 16S rRNA gene was performed. Demographics and dynamic variables of disease were recorded and compared between groups. 174 sputum samples (median 8, IQR (6-12)) from MABC cases and 42 control samples were assessed. The sputum microbiota from patients who would develop MABC infection in the subsequent two years differed from controls (p=0.038, R2 = 2.5%, PERMANOVA). In particular, sputum from MABC cases – prior to its identification – had higher alpha-diversity; Shannon diversity (p=0.023), Observed species (p=0.042) and lower P. aeruginosa relative and absolute abundance (p=0.035). We observed significant changes in community structure over time during potent antibacterial therapies, returning to baseline upon their discontinuation. These data suggest that sputum microbiome analysis and P. aeruginosa bioburden should be evaluated in multi-center studies as potential biomarkers to predict MABC infection and treatment response. | en_US |
| dc.identifier.citation | Bharadwaj, L. (2021). The Cystic Fibrosis Microbiome and its Association with Incident Infections with Mycobacteroides (Mycobacterium) abscessus (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. | en_US |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/38888 | |
| dc.identifier.uri | http://hdl.handle.net/1880/113441 | |
| dc.language.iso | eng | en_US |
| dc.publisher.faculty | Cumming School of Medicine | en_US |
| dc.publisher.institution | University of Calgary | en |
| dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | en_US |
| dc.subject | cystic fibrosis, Non-tuberculous Mycobacterium , pulmonary microbiome, 16S rRNA sequencing, sputum, | en_US |
| dc.subject.classification | Microbiology | en_US |
| dc.subject.classification | Health Sciences | en_US |
| dc.title | The Cystic Fibrosis Microbiome and its Association with Incident Infections with Mycobacteroides (Mycobacterium) abscessus | en_US |
| dc.type | master thesis | en_US |
| thesis.degree.discipline | Medicine – Microbiology & Infectious Diseases | en_US |
| thesis.degree.grantor | University of Calgary | en_US |
| thesis.degree.name | Master of Science (MSc) | en_US |
| ucalgary.item.requestcopy | true | en_US |