The Lyme disease spirochete can hijack the host immune system for extravasation from the microvasculature

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dc.contributor.authorTan, Xi
dc.contributor.authorPetri, Björn
dc.contributor.authorDeVinney, Rebekah
dc.contributor.authorJenne, Craig N
dc.contributor.authorChaconas, George
dc.date.accessioned2021-04-30T19:32:27Z
dc.date.available2021-04-30T19:32:27Z
dc.date.issued2021-04-23
dc.description.abstractLyme disease is the most common tick-transmitted disease in the northern hemisphere and is caused by the spirochete Borrelia burgdorferi and related Borrelia species. The constellation of symptoms attributable to this malady result from vascular dissemination of B. burgdorferi throughout the body to invade various tissue types. However, little is known about the mechanism by which the spirochetes can breach the blood vessel wall to reach distant tissues. We have studied this process by direct observation of spirochetes in the microvasculature of living mice using multilaser spinning-disk intravital microscopy. Our results show that in our experimental system, instead of phagocytizing B. burgdorferi, host neutrophils are involved in the production of specific cytokines that activate the endothelium and potentiate B. burgdorferi escape into the surrounding tissue. Spirochete escape is not induced by paracellular permeability and appears to occur via a transcellular pathway. Neutrophil repurposing to promote bacterial extravasation represents a new and innovative pathogenic strategy.en_US
dc.description.grantingagencyCanadian Institutes of Health Research (CIHR)en_US
dc.identifier.citationTan, X., Petri, B., DeVinney, R., Jenne, C. N., & Chaconas, G. (2021). The Lyme disease spirochete can hijack the host immune system for extravasation from the microvasculature. Molecular Microbiology.en_US
dc.identifier.doihttp://dx.doi.org/10.1111/mmi.14728en_US
dc.identifier.grantnumberPJT-153336en_US
dc.identifier.urihttp://hdl.handle.net/1880/113325
dc.language.isoengen_US
dc.publisherWileyen_US
dc.publisher.departmentBiochemistry & Molecular Biologyen_US
dc.publisher.departmentMicrobiology, Immunology & Infectious Diseasesen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.hasversionacceptedVersionen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.htmlen_US
dc.subjectBorreliaen_US
dc.subjectvascular transmigrationen_US
dc.subjectneutrophilsen_US
dc.subjectcytokinesen_US
dc.titleThe Lyme disease spirochete can hijack the host immune system for extravasation from the microvasculatureen_US
dc.typejournal articleen_US
ucalgary.item.requestcopytrueen_US
ucalgary.scholar.levelFacultyen_US
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