Inversion Modelling of Copper Transport in Saccharomyces Cerevisiae
Date
2020-05-12
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Abstract
Copper is an essential nutrient but the uptake into cells is poorly understood. This dissertation summarizes the development of a mathematical system of equations to model the transport of copper in S. cerevisiae. Yeast is a model organism for studying the copper transport in human hepatic cells because the chaperone proteins and structures are well conserved between the species. An experiment is performed to investigate the transport between the growth media and the cells to model the process behind this important pathway. Transport mechanisms for this process are presented, mathematically modelled, and evaluated. Rate limited diffusion did not appear to be adequate in modelling the transport, but a term including a target copper concentration which cells actively maintain was introduced, and with a delayed activation, fit the data much more effectively. With this model, a framework is established for incorporating organelles to eventually model the intracellular copper transport and analyze the copper isotope distributions in the future. This work contributes to a larger initiative to incorporate copper isotope analysis as an innovative medical diagnostic tool in assessing human cellular pathology.
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Analytical chemistry, Inversion problems, Inverse, Saccharomyces Cerevisiae, Copper, Stable Isotopes, Logistic, Isotope Dilution Mass Spectrometry, Mass Spectrometry, MC-ICP-MS, Modelling, Copper transport, Yeast, IDMS
Citation
Wilkins, A. F. (2020). Inversion Modelling of Copper Transport in Saccharomyces Cerevisiae (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.