Inversion Modelling of Copper Transport in Saccharomyces Cerevisiae

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dc.contributor.advisorWieser, Michael E.
dc.contributor.advisorKarchewski, Brandon
dc.contributor.authorWilkins, Aaron Francis
dc.contributor.committeememberDettmer, Jan
dc.contributor.committeememberStrous, Marc
dc.date2020-11
dc.date.accessioned2020-05-15T17:07:10Z
dc.date.available2020-05-15T17:07:10Z
dc.date.issued2020-05-12
dc.description.abstractCopper is an essential nutrient but the uptake into cells is poorly understood. This dissertation summarizes the development of a mathematical system of equations to model the transport of copper in S. cerevisiae. Yeast is a model organism for studying the copper transport in human hepatic cells because the chaperone proteins and structures are well conserved between the species. An experiment is performed to investigate the transport between the growth media and the cells to model the process behind this important pathway. Transport mechanisms for this process are presented, mathematically modelled, and evaluated. Rate limited diffusion did not appear to be adequate in modelling the transport, but a term including a target copper concentration which cells actively maintain was introduced, and with a delayed activation, fit the data much more effectively. With this model, a framework is established for incorporating organelles to eventually model the intracellular copper transport and analyze the copper isotope distributions in the future. This work contributes to a larger initiative to incorporate copper isotope analysis as an innovative medical diagnostic tool in assessing human cellular pathology.en_US
dc.identifier.citationWilkins, A. F. (2020). Inversion Modelling of Copper Transport in Saccharomyces Cerevisiae (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/37845
dc.identifier.urihttp://hdl.handle.net/1880/112058
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectAnalytical chemistryen_US
dc.subjectInversion problemsen_US
dc.subjectInverseen_US
dc.subjectSaccharomyces Cerevisiaeen_US
dc.subjectCopperen_US
dc.subjectStable Isotopesen_US
dc.subjectLogisticen_US
dc.subjectIsotope Dilution Mass Spectrometryen_US
dc.subjectMass Spectrometryen_US
dc.subjectMC-ICP-MSen_US
dc.subjectModellingen_US
dc.subjectCopper transporten_US
dc.subjectYeasten_US
dc.subjectIDMSen_US
dc.subject.classificationEducation--Mathematicsen_US
dc.subject.classificationEducation--Sciencesen_US
dc.subject.classificationBiophysicsen_US
dc.subject.classificationGeochemistryen_US
dc.subject.classificationChemistry--Analyticalen_US
dc.subject.classificationBiochemistryen_US
dc.subject.classificationPhysicsen_US
dc.subject.classificationPhysics--Molecularen_US
dc.subject.classificationApplied Sciencesen_US
dc.titleInversion Modelling of Copper Transport in Saccharomyces Cerevisiaeen_US
dc.typemaster thesisen_US
thesis.degree.disciplineGeoscienceen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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