Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis

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dc.contributor.authorShao, Shan
dc.contributor.authorWang, Yue
dc.contributor.authorZhao, Yan
dc.contributor.authorXu, Yuan
dc.contributor.authorWang, Tie
dc.contributor.authorDu, Kun
dc.contributor.authorBao, Shiping
dc.contributor.authorWang, Xiangdong
dc.contributor.authorZhang, Luo
dc.date.accessioned2022-06-12T00:03:32Z
dc.date.available2022-06-12T00:03:32Z
dc.date.issued2022-06-11
dc.date.updated2022-06-12T00:03:32Z
dc.description.abstractAbstract Background The conventional belief that glucocorticosteroid (GC) acts through a single brand glucocorticoid receptor (GR)α protein has changed dramatically with the discovery of multiple GR isoforms. We aimed to evaluate whether multiple GR protein isoforms are expressed in chronic rhinosinusitis with nasal polyps (CRSwNP) and whether GR protein isoform expression profiles differ between different endotypes of CRSwNP. Methods Thirty-eight patients with CRSwNP and ten healthy volunteers were included. The protein expression of multiple GR isoforms in nasal polyps (NPs) tissue and control mucosae was examined by western blot analysis with different GR antibodies. Results Five bands, including three bands for known proteins (GRα-A/B, GRα-C, and GRα-D) and two bands for unidentified proteins at 67 kilodaltons (kDa) and 60 kDa, were identified with both total GR antibody (PA1-511A) and GRα-specific antibody (PA1-516). GRα-D intensity, which was abundant in nasal mucosa, was significantly increased in the CRSwNP group and was especially elevated in the noneosinophilic CRSwNP (NE-CRSwNP) group (PA1-511A: P < 0.001 and P = 0.0018; PA1-516: P < 0.003 and P = 0.006, respectively). Additionally, the intensities of the newly recognized 67 kDa and 60 kDa bands were much greater in the NE-CRSwNP subgroup than in the eosinophilic CRSwNP (E-CRSwNP) subgroup; in the E-CRSwNP subgroup, the median intensities were even lower than those in the control group. Conclusions This study provides evidence that nasal tissues express multiple GR protein isoforms. GR protein isoforms presented disease and tissue-specific expression profiles that differed between the CRSwNP and control groups and between the E-CRSwNP and NE-CRSwNP subgroups. Graphical abstract
dc.identifier.citationJournal of Otolaryngology - Head & Neck Surgery. 2022 Jun 11;51(1):25
dc.identifier.doihttps://doi.org/10.1186/s40463-022-00561-1
dc.identifier.urihttp://hdl.handle.net/1880/114724
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.titleIdentification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis
dc.typeJournal Article
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