Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC
| dc.contributor.author | Garcia-Caballero, Agustin | |
| dc.contributor.author | Gandini, Maria A | |
| dc.contributor.author | Huang, Shuo | |
| dc.contributor.author | Chen, Lina | |
| dc.contributor.author | Souza, Ivana A | |
| dc.contributor.author | Dang, Yan L | |
| dc.contributor.author | Stutts, M. J | |
| dc.contributor.author | Zamponi, Gerald W | |
| dc.date.accessioned | 2019-02-10T01:03:55Z | |
| dc.date.available | 2019-02-10T01:03:55Z | |
| dc.date.issued | 2019-02-08 | |
| dc.date.updated | 2019-02-10T01:03:55Z | |
| dc.description.abstract | Abstract This study describes the functional interaction between Cav3.2 calcium channels and the Epithelial Sodium Channel (ENaC). β-ENaC subunits showed overlapping expression with endogenous Cav3.2 calcium channels in the thalamus and hypothalamus as detected by immunostaining. Moreover, β- and γ-ENaC subunits could be co-immunoprecipitated with Cav3.2 calcium channels from brain lysates, dorsal horn and lumbar dorsal root ganglia. Mutation of a cluster of lysines present in the intracellular N-terminus region of β-ENaC (K4R/ K5R/ K9R/ K16R/ K23R) reduced interactions with Cav3.2 calcium channels. Αβγ-ENaC channels enhanced Cav3.2 calcium channel trafficking to the plasma membrane in tsA-201 cells. This effect was reciprocal such that Cav3.2 channel expression also enhanced β-ENaC trafficking to the cell surface. T-type current density was increased when fully assembled αβγ-ENaC channels were transiently expressed in CAD cells, a neuronal derived cell line. Altogether, these findings reveal ENaC as an interactor and potential regulator of Cav3.2 calcium channels expressed in neuronal tissues. | |
| dc.identifier.citation | Molecular Brain. 2019 Feb 08;12(1):12 | |
| dc.identifier.doi | https://doi.org/10.1186/s13041-019-0433-8 | |
| dc.identifier.uri | http://hdl.handle.net/1880/109896 | |
| dc.language.rfc3066 | en | |
| dc.rights.holder | The Author(s). | |
| dc.title | Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC | |
| dc.type | Journal Article |