The immediately releasable pool of mouse chromaffin cell vesicles is coupled to P/Q-type calcium channels via the synaptic protein interaction site

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dc.contributor.authorÁlvarez, Yanina D.
dc.contributor.authorBelingheri, Ana Verónica
dc.contributor.authorPerez Bay, Andrés E.
dc.contributor.authorJavis, Scott E.
dc.contributor.authorTedford, Hugo William
dc.contributor.authorZamponi, Gerald W.
dc.contributor.authorMarengo, Fernando D.
dc.date.accessioned2018-05-29T19:09:57Z
dc.date.available2018-05-29T19:09:57Z
dc.date.issued2013-01-30
dc.description.abstractIt is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca(2+) channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca(2+) current. Accordingly, in the present work we found that the Ca(2+) current flowing through P/Q-type Ca(2+) channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca(2+) current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K(+) stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca(2+) channels.en_US
dc.identifier.citationÁlvarez, Y. D., Belingheri, A. V., Perez Bay, A. E., Javis, S. E., Tedford, H. W., Zamponi, G., & Marengo, F. D. (2013). The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site. PLoS ONE, 8(1). https://doi.org/10.1371/journal.pone.0054846en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0054846en_US
dc.identifier.urihttp://hdl.handle.net/1880/106705
dc.language.isoenen_US
dc.publisherPLOS oneen_US
dc.publisher.departmentPhysiology & Pharmacologyen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleThe immediately releasable pool of mouse chromaffin cell vesicles is coupled to P/Q-type calcium channels via the synaptic protein interaction siteen_US
dc.typejournal articleen_US
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