Integration of deep transcriptome and proteome analyses reveals the components of alkaloid metabolism in opium poppy cell cultures
Date
2010-11-18
Journal Title
Journal ISSN
Volume Title
Publisher
BMC Plant Biology
Abstract
Papaver somniferum (opium poppy) is the source for several pharmaceutical benzylisoquinoline alkaloids including morphine, the codeine and sanguinarine. In response to treatment with a fungal elicitor, the biosynthesis and accumulation of sanguinarine is induced along with other plant defense responses in opium poppy cell cultures. The transcriptional induction of alkaloid metabolism in cultured cells provides an opportunity to identify components of this process via the integration of deep transcriptome and proteome databases generated using next-generation technologies.
Description
Keywords
Berberine Bridge Enzyme, Papaver-Somniferum, Molecular-Cloning, Morphine Biosynthesis, Dihydrobenzophenanthridine Oxidase, Norcoclaurine Synthase, N-Methyltransferase, Suspension Cultures, Gene Transcript, Expression
Citation
Desgagné-Penix, I., Khan, M. F., Schriemer, D. C., Cram, D., Nowak, J., & Facchini, P. J. (2010). Integration of deep transcriptome and proteome analyses reveals the components of alkaloid metabolism in opium poppy cell cultures. BMC Plant Biology, 10(1), 252-252. doi:10.1186/1471-2229-10-252